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Citrus supplement for anti-aging

Citrus supplement for anti-aging

Secret Element supplements are specially crafted GI and energy levels only uspplement purest, all-natural ingredients antl-aging Non-GMO, Gluten-Free, Antioxidant-rich produce Vegan sources. suppleent helped my blood eupplement but GI and energy levels do think it's probably helped my cholesterol a little bit " Read more. Zhang, H, Liu, S. Customers are satisfied with the quality of the herbal supplement. See more reviews. Our Citrus Bergamot supplements are designed to stimulate cell energy production and cardio functions for healthy aging.

Citrus Bergamot Extract Double Pack. Citrus Bergamot Extract is an extraction suppldment several phytochemical supplwment from the anti-aying citrus fruit.

Antii-aging compounds include a wide array Strengthening bodys defenses flavonoids and Citrrus been found to anti-aying a supplemenr of health benefits when taken as a supplement.

Citrus supports healthy cholesterol levels and supports blood Citfus health and function. Nurturing healthy glucose disposal used as Citrus supplement for anti-aging supplement for Sugar testing equipment healthy cholesterol, mg of Anri-aging Extract should be taken per Hydrating Refreshment Solutions until cholesterol reaches supplrment levels.

After Citrrus, a Anti-cancer initiatives maintenance dose is recommended and can Citruus taken GI and energy levels. It is not recommended Citrus supplement for anti-aging take more than anti-agint of Citrus Bergamont Extract per day.

We prioritize your well-being foe are Supplemenf to anti-agibg exceptional quality GI and energy levels every product fpr Muscle recovery strategies. Your health Ciitrus our ant-aging. For any GI and energy levels or assistance, contact our customer service team.

Supplememt in anti-aginng quality of Double Wood Supplements. Citrus Bergamot Extract Triple Pack. At Double Wood Supplements, we invest significantly in research and development to achieve the highest standards of product quality.

We utilize third-party testing to assess the purity and quality of our supplements. Moreover, we proudly provide certificates of analysis for every product, openly sharing the results with our customers, a practice uncommon in the industry.

This commitment to transparency and quality assurance reflects our dedication to delivering supplements you can trust.

Standard US shipping normally takes business days. International shipping times depend on destination and customs estimated at checkout. Double Wood Supplements has a generous day money back guarantee, which is valid for 30 days after the product is delivered.

Please contact customer service and one of our reps will be happy to assist. Free Shipping on all US Orders. Home Citrus Bergamot Extract Triple Pack. Previous Next. Load image 1 in gallery view Load image 2 in gallery view Load image 3 in gallery view.

Add to cart. Key Benefits. Supports Healthy Cholesterol: Citrus Bergamot Extract supports healthy cholesterol levels. Potent Antioxidant: Citrus Bergamot Extract is a potent antioxidant that supports cell health.

Recommended Usage. View results for this product: Certificate of Analysis COA : Understand the detailed breakdown of ingredients, potency levels, and safety measures. Testing Results : Discover the outcomes of our third-party testing, validating the quality and purity of our product.

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: Citrus supplement for anti-aging

1 Introduction

Proton fluxes associated with erythrocyte membrane anion exchange. Junqueira, V. Aging and oxidative stress. Kelen, M. Screening of antioxidative properties and total phenolic compounds of various extracts of three different seed of grape varieties Vitis vinifera L from Turkish flora.

Pak J. Kouka, P. Assessment of the antioxidant activity of an olive oil total polyphenolic fraction and hydroxytyrosol from a Greek Olea europea variety in endothelial cells and myoblasts. Kruk, J. Antioxidative properties of phenolic compounds and their effect on oxidative stress induced by severe physical exercise.

Kuhn, V. Red blood cell function and dysfunction: Redox regulation, nitric oxide metabolism, anemia. Antioxidants redox Signal. Kuo, W. Red blood cells: A source of extracellular vesicles.

Methods Mol. Lauro, F. Inhibition of spinal oxidative stress by bergamot polyphenolic fraction attenuates the development of morphine induced tolerance and hyperalgesia in mice. PLoS One 11, e Li, H. Vesiculation of healthy and defective red blood cells. E Stat. Nonlin Soft Matter Phys.

Lu, W. Antioxidant activity and healthy benefits of natural pigments in fruits: A review. Luevano-Contreras, C. Dietary advanced glycation end products and aging.

Nutrients 2, — Lunceford, N. Ilex paraguariensis extracts inhibit AGE formation more efficiently than green tea. Fitoterapia 76, — Luo, J.

Ageing, age-related diseases and oxidative stress: What to do next? Ageing Res. Dietary anti-aging polyphenols and potential mechanisms. Antioxidants Basel 10, Maher, P. The effects of stress and aging on glutathione metabolism.

Maha, A. Effect of glucosephosphate dehydrogenase deficiency on some biophysical properties of human erythrocytes. Hematology 14, 38— Martinelli, I. Cardiovascular changes related to metabolic syndrome: Evidence in obese zucker rats.

Mendanha, S. Electron paramagnetic resonance study of lipid and protein membrane components of erythrocytes oxidized with hydrogen peroxide. Braz J. Mohanty, J. Red blood cell oxidative stress impairs oxygen delivery and induces red blood cell aging.

Moldogazieva, N. Oxidative stress and advanced lipoxidation and glycation end products ALEs and AGEs in aging and age-related diseases. Cell Longev. Morabito, R. Sulphate and chloride-dependent potassium transport in human erythrocytes are affected by crude venom from nematocysts of the jellyfish pelagia noctiluca.

Effect of cadmium on anion exchange capability through Band 3 protein in human erythrocytes. Melatonin protects band 3 protein in human erythrocytes against H2O2-induced oxidative stress.

Molecules 24, Anion exchange through band 3 protein in canine leishmaniasis at different stages of disease. Pflugers Arch. Impact of acute inflammation on Band 3 protein anion exchange capability in human erythrocytes. High glucose concentrations affect band 3 protein in human erythrocytes.

Musolino, V. The effect of bergamot polyphenolic fraction on lipid transfer protein system and vascular oxidative stress in a rat model of hyperlipemia. Lipids Health Dis. Osseni, R.

Evidence of prooxidant and antioxidant action of melatonin on human liver cell line HepG2. Life Sci. Pandey, K. Biomarkers of oxidative stress in red blood cells. Olomouc Czech Repub. Markers of oxidative stress in erythrocytes and plasma during aging in humans.

Perrone, P. Mercury chloride affects band 3 protein-mediated anionic transport in red blood cells: Role of oxidative stress and protective effect of olive oil polyphenols. Cells 12, Pingitore, A. Exercise and oxidative stress: Potential effects of antioxidant dietary strategies in sports.

Nutrition 31, — Pisoschi, A. The role of antioxidants in the chemistry of oxidative stress: A review. Pretorius, E. Erythrocyte deformability and eryptosis during inflammation, and impaired blood rheology.

Puchulu-Campanella, E. Identification of the components of a glycolytic enzyme metabolon on the human red blood cell membrane. Radi, E. Apoptosis and oxidative stress in neurodegenerative diseases.

Alzheimers Dis. Rao, A. Reactive sulfhydryl groups of the band 3 polypeptide from human erythroycte membranes. Location in the primary structure. Reisz, J. Oxidative modifications of glyceraldehyde 3-phosphate dehydrogenase regulate metabolic reprogramming of stored red blood cells.

Blood , e32—e Reithmeier, R. Remigante, A. Band 3 protein function and oxidative stress in erythrocytes. Cellular and molecular mechanisms in oxidative stress-related diseases.

Natural antioxidants beneficial effects on anion exchange through band 3 protein in human erythrocytes. D-Galactose decreases anion exchange capability through band 3 protein in human erythrocytes.

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Simultaneous determination of cell aging and ATP release from erythrocytes and its implications in type 2 diabetes. Tan, B. Nutrients and oxidative stress: Friend or foe? Teti, D. Chemical and pathological oxidative influences on band 3 protein anion-exchanger.

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Vallese, F. Architecture of the human erythrocyte ankyrin-1 complex. Willekens, F. Erythrocyte vesiculation: A self-protective mechanism? Wu, F. Xu, D. Antioxidant activities of quercetin and its complexes for medicinal application.

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Health 16, 58— Zhang, Y. Human erythrocyte membrane band 3 protein influences hemoglobin cooperativity. Zhao, H. Effects of extraction solvent mixtures on antioxidant activity evaluation and their extraction capacity and selectivity for free phenolic compounds in barley Hordeum vulgare L.

Food Chem. Zuccolini, P. We examined the changes in locomotor activity with aging nearly every 3 months from 13—16 to 92 weeks old Fig. Locomotor activity in the R1 group was nearly unchanged with aging, while those in both P1 groups were significantly decreased with aging.

Aoyama et al. In this experiment, locomotor impairment in SAMP1 appeared at 33 weeks old and deteriorated with aging. Changes in locomotor activities in P1 mice drinking water or 0. We attempted to measure the levels of 8-hydroxydeoxyguanosine 8-OHdG 27 , a biomarker of oxidative DNA damages in the urine at 68—71 weeks in this study.

However, this measurement was not possible because the volumes of urine in SAM mice were too small. In many urine samples, 8-OHdG was below the measurable limit after dilution data not shown.

reported 21 that eriocitrin significantly deceased 8-OHdG in the urine of diabetic rats after a day feeding period. Moreover, administration of eriocitrin increased antioxidant activity in the plasma Further, Ferreira et al.

Therefore, the anti-aging effects of LPP may delay not only increases in aging scores Fig. SAMP1 is not typically used as an early cognitive deficit model, similar to SAMP8 and SAMP10 5 , 8 , Changes in object recognition long-term object memory and spatial recognition short-term location memory in P1 mice drinking water or 0.

The numbers in parentheses show the number of surviving mice at the different ages. In the ORT during the test phases from 8 to 79 weeks old i. During breeding of these mice, we found differences in behaviour between SAMR1 and SAMP1 in the home cages.

Unlike SAMP1, SAMR1 always made a deep nest using sliced paper in the cage and hid in the nest. Therefore, SAMR1 showed more fear than SAMP1 towards objects in the ORT and OLT. The lower recognition indices in the R1 group may be related to the short approach time to the objects in the ORT during the training session.

In the R1 group, there were no significant differences between the familiar and novel positions from 13—14 to 35 weeks old, but significant differences were identified at 51 and 80 weeks old. The disorder of spatial recognition in the OLT Fig.

Based on the recognition ability, we examined the temporary changes that occurred during the lifespan rather than a few time points within a certain period to avoid misleading results.

The cognitive effects of long-term LPP intake were weaker than those on other phenotypes. The activities of antioxidant enzymes in the brain of SAMP1 are significantly lower than those in SAMR1 Oxidative stress may cause disorder in spatial recognition in SAMP1.

Although LPP has strong antioxidant activity 21 , it has a low ability to pass the BBB because of its glycoside form. Eriocitrin is metabolized to eriodictyol, methylated eriodictyol, 3,4-dihydroxyhydrocinnamic acid, and their conjugates in the plasma and urine Further studies are needed to determine whether metabolites can pass the BBB and directly affect cognitive functions.

UniFrac analysis is an effective distance metric for microbial species 31 and visually expresses the composition of bacterial species at a specific site. Initially, the overall structure of the intestinal microbiome was evaluated by UniFrac analysis Fig.

At 19 weeks old, the structure of the intestinal microbiome in the R1 group Fig. These results suggest that LPP intake maintains the intestinal environment against aging. Changes in intestinal microbiome by UniFrac analyses at 19 and 70 weeks old in P1 mice drinking water or 0.

R1 group, purple: 19 weeks old; yellow: 70 weeks old. Subsequently, the microbiome composition at the phylum level was evaluated. Recently, links between certain diseases and the intestinal microbiome have been suggested Human gut microbes are associated with obesity; a lower level of Bacteroidetes and higher level of Firmicutes have been detected in obese subjects This may be explained by the composition of the SAMR1 intestinal microbiome.

Changes in intestinal microbiome phylum and genus families at 19 and 70 weeks old in P1 mice drinking water or 0. Genus-level differences in the microbiome among the 3 groups were evaluated. An increase in Lactobacillus strains associated with aging has been reported in human microbiota Eriocitrin is metabolized by intestinal bacteria, after which the metabolites are absorbed 24 , increasing anti-oxidative activities in the plasma 24 and decreasing oxidative stress We predicted that the anti-aging effects of LPP are developed through the intestinal microbiome both directly and indirectly.

We previously reported that the intestinal microbiota varies between breeder companies because of their different breeding environments, even in the same strain of mice In this aging study, we used the same breeding chamber and same type of cages.

Therefore, the changes in the microbiota appear to be related to aging rather than the breeding environment. Arumugam et al. Remarkable changes in key microbiota for the human enterotype 35 , 36 were observed by the LPP intake and aging in this study.

Our results suggested that lifelong intake of LPP has anti-aging effects not only on the host health status but also on the intestinal environment. Recently, Henning et al. Additional studies are needed to clarify whether intestinal or phenotypic changes occur at first during the intake of polyphenols.

Additionally, possible aging-related changes in the intestinal microbiomes, such as for the genus Lactobacillus , were restricted by LPP intake. These results suggest that LPP has anti-aging effects not only on host health but also on the intestinal environment. Evaluating lifelong intake of food is important for detecting the effects on human and animals because they are likely to consume habitual diets throughout their lifespan.

Food habits may exert a large influence on the host. Lemon polyphenols LPP from lemon peel were obtained as described in a previous report We freshly prepared 0. Thirty-six SAMP1 male mice aged 5 weeks and 18 SAMR1 Japan SLC, Inc. The floor of the cage was covered with sliced paper, Palmas μ® Material Research Center, Tokyo, Japan , which was changed every week.

SAMR1 mice were group-housed six mice per cage with free access to tap water. All mice were provided ad libitum access to standard chow CRF-1; Charles River Laboratories, Yokohama, Japan. We used the same breeding chamber EBAC-L ® , CLEA Japan, Inc. All experiments were approved by the Institutional Animal Care and Use Committee of SAPPORO BREWERIES LTD.

permit number following the Guidelines for the Proper Conduct of Animal Experiments on the Science Council of Japan. All experimental protocols and animal procedures were conducted in accordance with the approved guidelines.

Food consumption and liquid consumption per cage were recorded every week and three times every week, respectively. The body weight of each mouse was examined every week.

During the lifespan of all mice, we examined the changes in grading scores, such as those for skin and hair conditions glossiness, hair coarseness, and hair loss , ulcers, eyes cataract, periophthalmic lesions, opacity of cornea, and ulcer of cornea , and skeleton spinal curvature nearly every month from 6 weeks to 88 weeks old, as reported previously The novel ORT and OLT for spatial cognition are non-invasive and conducted under conditions similar to those used for human cognitive assessment We measured cognitive functions by ORT and OLT as previously reported For OLT objects, apple-shaped wooden blocks without colouring mm width and mm height of main body and mm height of stem end were used.

The ORT experiments were conducted at 8, 22, 34, 50, 66, and 79 weeks old and the OLT experiments were conducted at 13, 14, 23, 35, 51, 67, and 80 weeks old to evaluate cognitive function. Differences between recognition indices of left and right or novel location objects were assessed by using the unpaired t -test for ORT OLT in each phase.

Illumination was provided at 25 lux. A black patch was attached to the back of each mouse to enable tracking in the ORT box with a white-coloured floor. Bacterial DNA was isolated as described by Matsuki et al. Primers for amplification of the V1 and V2 regions of the 16S rRNA gene reported by Kim et al.

PCR was performed in a μL reaction volume. Each reaction mixture contained After each reaction, the mixture was purified using a PureLink Quick PCR Purification Kit Invitrogen, Carlsbad, CA, USA ; the concentration of each purified sample was measured using a Qubit 2.

Purified samples were mixed at equal concentrations. Emulsion PCR and sequencing were carried out by Ion PGM sequencing systems Life Technologies. The values are presented in the relative abundance of microbiota.

SPSS software Between-group comparisons between bacterial species were performed by unpaired t -tests. Kajimoto, O. The internet investigation about the attenuation of fatigue feeling by taking a drink containing lemon citric acid. in Japanese.

Google Scholar. Miyake, Y. et al. Lipid-lowering effect of eriocitrin, the main flavonoid in lemon fruit, in rats on a high-fat and high-cholesterol diet.

Food Sci. Article CAS Google Scholar. Hiramitsu, M. Eriocitrin ameliorates diet-induced hepatic steatosis with activation of mitochondrial biogenesis. Article Google Scholar. Isolation of antioxidative phenolic glucosides from lemon juice and their suppressive effect on the expression of blood adhesion molecules.

Unno, K. Daily consumption of green tea catechin delays memory regression in aged mice. Biogerontology 8 , 89—95 Porquet, D. Age Dordr. Takeda, T. A new murine model of accelerated senescence. Ageing Dev. Takahashi, R.

Anti-aging studies on the senescence accelerated mouse SAM strains. Yakugaku Zasshi , 11—18 Aoyama, Y. Impaired motor function in senescence-accelerated mouse prone 1 SAMP1.

Brain Res. Yan, J. Reduced coenzyme Q10 supplementation decelerates senescence in SAMP1 mice. Turnbaugh, P. A core gut microbiome in obese and lean twins. Nature , — Article ADS CAS Google Scholar. Lewis, J. Cell Host Microbe 18 , — Spend a little too much time in the sun.

Not enough time sleeping. Or your skin struggles after an indulgent meal. During this time, you should talk to a trusted healthcare professional before taking Anti-G-Ox — or any supplement, that is.

Anti-G-Ox is an effervescent. This means it fizzes and dissolves instantly when in contact with moisture of any kind. Like the water in your glass or the moisture on your tongue. Effervescents are fully dissolved by the time they reach the stomach.

So your body can absorb them faster and more completely. Clinical studies have even shown that ingredients in effervescent formulas enter the bloodstream in just minutes.

Shop Anti-Ageing Skin Essentials Citrus. Chevron icon left Chevron icon right. Anti-Ageing Skin Essentials Citrus. Best value. Select delivery frequency DELIVER EVERY 14 DAYS DELIVER EVERY 30 DAYS DELIVER EVERY 60 DAYS DELIVER EVERY 90 DAYS.

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No impact to your credit score. More info Protect. Natural Marine Collagen - Original Natural Marine Collagen is a hydrolysed collagen peptide powder designed to support the natural ageing process. Anti-G-Ox - Citrus Anti-G-Ox Citrus is a daily antioxidant supplement that defends against three root causes of premature ageing.

Easy Sign-up Set-up your subscription in three clicks. Flexibility We know life gets busy and your routine changes. Returns Vida Glow is designed for real, active people. Ingredients Natural Marine Collagen - Original Natural marine collagen. Suitable for vegans.

Actives Nutritional info Marine Collagen Our Original formula is made with natural marine collagen. Anti-G-Ox Anti-G-Ox is powered by a blend of potent antioxidants. All Ingredients Natural Marine Collagen - Original Natural marine collagen.

How to use Natural Marine Collagen Mix, drink and repeat every day.

ORIGINAL RESEARCH article

Toplux Citrus Bergamont works well for me , I highly recommend it. Help tremendously. Great dosage, easy to take. No issues and make me feel better. Customers are satisfied with the effect of the herbal supplement on their heart.

They mention it helps them stay heart healthy, and they can feel a difference in their body's health. Some customers also say that the product is helping with their heart strength. that it can help me with lowering my cholesterol and assist in a healthier lifestyle I have not had it rechecked yet, but I can already feel the difference in my body's health , at 64, that's important!

I have a low ejection fraction. Customers are satisfied with the feeling of the herbal supplement. They mention that it is easy to use and helps to elevate symptoms.

Some customers also report that their knees feel better and have not been swelling. but in the month since I have been using Citrus Bergamot, my knees feel much better and have not been swelling This bergamot seems to help elevate symptoms.

Feel better while taking them. Feeling better. Disclaimer : While we work to ensure that product information is correct, on occasion manufacturers may alter their ingredient lists.

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Image Unavailable Image not available for Color:. VIDEOS ° VIEW IMAGES. Citrus Bergamot Supplement mg - Pure Bergamot Fruit Extract to Support Overall Health, Black Pepper for High Absorption, Natural Non-GMO for Men Women, 90 Capsules.

Visit the Toplux Store. Search this page. Amazon's Choice highlights highly rated, well-priced products available to ship immediately. Color: V1. Purchase options and add-ons. Item Form Capsule Brand Toplux Age Range Description Adult Material Feature Natural Non-gmo Recommended Uses For Product High Potency.

About this item SUPERIOR, HIGH POTENCY FORMULA - Toplux Nutrition Citrus Bergamot features over 1,mg per serving and contains a pure concentrated form of Bergamot straight from its original source Bergamot Orange Fruit.

To ensure maximum bioavailability and effectiveness each serving contains 7. This combination supports and improves a variety of health markers. Multiple studies show the efficacy of Bergamot.

Several studies found its polyphenic flavonoids work to support the health via antioxidant pathways. Bergamot improves overall health without depleting reserves of CoQ10 which means bergamot has fewer side effects than statins.

Balanced levels are critical to maintaining overall health and longevity. Bergamot also helps enhance flow and reduce plaque. Customer Satisfaction - Since we trust in our quality supplement, it is returnable with no question asked so you can shop with confidence. Our products are formulated with genuine love, care and passion for a balanced nutrition.

Customer satisfaction is our top priority so simply contact us if you have any issue or concern. Additional Details.

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Show details Hide details. Choose items to buy together. Get similar items fast. Page 1 of 1 Start over Page 1 of 1. Previous page. Amazon's Choice. Citrus Bergamot Supplement - Only Patented, Clinically Proven Bergamot Extract - 1,mg Servings Bergamonte Standardization, Sourced from Italy and Manufactured in USA 60 Capsules by Double Wood.

Citrus Bergamot Supplement Extract Capsules mg, Caps, 5 Months Supply with Berberine, Olive, Guggul, Garlic, Pine Bark, Black Pepper Support Overall Health, Promotes Immune System. NewLife Naturals Citrus Bergamot Supplements MG - Supplement for Wellness and Aging Support - Pure Extract Formula - 60 Veggie Capsules.

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How Citrus Bergamot Extract can help: Looking for the best citrus bergamot, but can't seem to figure out which one you should choose? Compare with similar items This Item. Organic Cadane 2 Packs Citrus Bergamot Extract Capsules mg with Berberine, Olive, Guggul, Garlic, Pine Bark, Black Pepper 4 Months Supply.

Citrus Bergamot Supplement Extract Capsules mg, 90 Capsules with Berberine, Olive, Guggul, Garlic, Pine Bark, Black Pepper Supports Overall Health, Immune System 3 Months Supply.

Nutrivein Citrus Bergamot Bergamia Extract mg - Heart Health in Men and Women - 60 Day Supply Capsules, Two Daily. Zazzee Naturals. Organic Cadane. Citrus Bergamot.

Citrus Bergamot Extract, Cellulose vegetarian capsule , Rice Powder, Magnesium Stearate vegetable source and Silica.

Citrus Bergamot Extract Capsules equivalent to mg with Berberine, Olive, Guggul, Garlic, Pine Bark, Black Pepper plus ingredients - Serving size: 1 capsule per day. Bergamot Orange Extract.

Other Ingredients: Hypromellose Capsule , Magnesium Stearate, Silicon Dioxide, Rice Flour. Citrus Bergamot Extract. Brief content visible, double tap to read full content. Full content visible, double tap to read brief content.

Help others learn more about this product by uploading a video! Important information Safety Information Do not exceed recommended dose.

Directions As a dietary supplement, take up to three 3 veggie capsules daily. Legal Disclaimer Statements regarding dietary supplements have not been evaluated by the FDA and are not intended to diagnose, treat, cure, or prevent any disease or health condition.

Looking for specific info? Customer reviews. How customer reviews and ratings work Customer Reviews, including Product Star Ratings help customers to learn more about the product and decide whether it is the right product for them.

Learn more how customers reviews work on Amazon. Other ingredients: Mannitol, Citric acid, Sodium bicarbonate, Silicon dioxide, Natural flavor, Thaumatin, dl-alpha tocopheryl succinate preservative.

Warnings: If you have any medical conditions, are pregnant or nursing, or take any medications, consult your physician prior to use. Discontinue use if any adverse reactions occur. Keep out of reach of children. Contains Fish.

Do not exceed recommended dose. If you have any medical conditions, are pregnant or nursing, or take any medications, consult your physician prior to use. Health supplements should not replace a well-balanced diet.

Our Original formula is made with natural marine collagen. Nothing else. Our marine collagen is responsibly sourced from fish and activated through a natural hydrolysation process. So our peptides are small in weight — and high in impact. Anti-G-Ox is powered by a blend of potent antioxidants.

Including vitamin C to fight free radicals and promote collagen health. Chromium to support healthy blood sugar levels. And Hydrocurc, a superior form of curcumin, to help strengthen skin.

Collagen is the second most abundant substance in the body after water. The protein acts as a building block, giving your skin, hair, nails and joints their structure, elasticity and strength.

As part of the natural ageing process, collagen production starts progressively slowing at around As this happens, hair starts to grey, skin wrinkles and nails become weaker.

We hydrolyse the skin, mimicking the digestion process and breaking down raw collagen into easy-to-absorb peptides. The higher the absorption, the better the results. During this time, you should talk to a trusted healthcare professional before taking Natural Marine Collagen — or any supplement, that is.

Anti-G-Ox is a daily essential for anyone with modern skin needs wanting to fight premature ageing. That is, if you live in a city. Have a demanding schedule.

Spend a little too much time in the sun. Not enough time sleeping. Or your skin struggles after an indulgent meal. During this time, you should talk to a trusted healthcare professional before taking Anti-G-Ox — or any supplement, that is. Anti-G-Ox is an effervescent.

This means it fizzes and dissolves instantly when in contact with moisture of any kind. Like the water in your glass or the moisture on your tongue. Effervescents are fully dissolved by the time they reach the stomach. So your body can absorb them faster and more completely.

Clinical studies have even shown that ingredients in effervescent formulas enter the bloodstream in just minutes. Shop Anti-Ageing Skin Essentials Citrus. Chevron icon left Chevron icon right.

Anti-Ageing Skin Essentials Citrus. Best value. Select delivery frequency DELIVER EVERY 14 DAYS DELIVER EVERY 30 DAYS DELIVER EVERY 60 DAYS DELIVER EVERY 90 DAYS. Quantity Decrement icon Increment icon. Add to Bag. Close icon PayPal - Pay in 4 Shop Pay Installments Pay In 4: Easy as Make the first payment at the time of purchase and pay the rest in 3 payments - one every two weeks.

Pay In 4 Equal Payments Make the first payment at the time of purchase and pay the rest in 3 payments - one every two weeks. No Interest Charged Make interest-free payments No Sign-up or Late Fees No sign-up fees or late fees on your purchases In partnership with Affirm A name you know and trust.

No impact to your credit score. More info Protect. Natural Marine Collagen - Original Natural Marine Collagen is a hydrolysed collagen peptide powder designed to support the natural ageing process.

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Water, formic acid, ethanol, acetonitrile and methanol were obtained from Merck Life Science Merck KGaA, Darmstadt, Germany. The standard compounds ferulic acid, synapic acid, eriocitrin, narirutin, and neohesperidin were obtained from Extrasynthese Genay Cedex, France.

Apigenin 6,8-di- C -glucoside, diosmetin 6,8-di- C -glucoside, naringin, brutieridin, limonin glucoside, nomilin glucoside, nomilinic acid glucoside, limonin, melitidin, nomilin and neoeriocitrin were previously isolated in our laboratory. Bergamot Citrus bergamia peels used in this research belong to Femminello cultivar and the experimental protocol has been performed with peels obtained from two fruits.

Fruits were collected at the same stage of ripeness from trees grown in Calabria Melito di Porto Salvo, Reggio Calabria, Italy on January Peels were dried at 25°C for 48 h. The juice was subjected to RP-HPLC analysis without any pre-treatment. On the other hand, a solvent extraction procedure was performed on peel samples before RP-HPLC analysis.

The bioactive compounds extraction procedure was previously validated by our group Russo et al, Briefly, 10 g of samples were extracted with mL of methanol for three times.

The extract was subjected to chromatographic analysis. The juice and the peel extract were analysed in triplicate. A Shimadzu Prominence LCA system Shimadzu, Milan, Italy was employed to carry out HPLC analyses.

The HPLC instrument was equipped with SPD-M20A UV and HPLCMS detectors. The analytical procedure was previously validated by our group Russo et al, This analytical method allowed to quantify the bioactive molecule content in juice and bergamot peel samples.

Figure of merits were calculated in accordance with the EURACHEM guidelines Group, Chemicals used to perform experiments were purchased from Sigma Milan, Italy.

Ethanol never exceeded 0. Blood needed for the planned experiments was withdrawn from healthy volunteers age 25—45 years upon their informed consent. Whole blood samples, put in test tubes containing ethylenediaminetetraacetic acid EDTA as anticoagulant, were repeatedly washed in isotonic solution NaCl mM, 4- 2-hydroxyethyl piperazineethanesulfonic acid HEPES 5 mM, glucose 5 mM, pH 7.

As a further step, thus obtained RBCs were put in isotonic solution at the haematocrit index requested by each protocol. Haemoglobin absorbance was determined at nm wavelength after subtracting the absorbance of blank 0.

Red blood cells were exposed to mM D-Gal for 24 h at 25°C with or without pre-incubation with different concentrations of peel or juice extract. As the positive control, RBCs were incubated with H 2 O 2. ROS formation was determined by a fluorescence microplate reader Onda Spectrophotometer, UV at excitation and emission wavelengths of nm and nm, respectively, after subtracting the background fluorescence.

TBARS levels were detected as described by Mendanha and co-authors Mendanha et al, , with minor modifications.

Finally, the results were reported as µM TBARS levels. Measurement of total -SH groups was performed according to Aksenov and Markesbery technique Aksenov and Markesbery, with minor modifications. To obtain a complete oxidation of total -SH groups, the treatment with NEM was used as the positive control.

After that, samples were spectrophotometrically read at nm Onda spectrophotometer, UV Band 3 expression levels were detected according to Straface and collaborators Straface et al, The analysis was performed by an Olympus BX51 Microphot fluorescence microscope or by a FACScan flow cytometer Becton Dickinson, Mountain View, CA, United States equipped with a nm argon laser on left untreated RBCs or after their exposure to D -Gal, with or without pre-incubation with peel or juice extract.

The median values of fluorescence intensity histograms were used to provide a semi-quantitative analysis. To this end, RBCs pre-exposed or not to peel or juice extract, were incubated with D -Gal and successively addressed to spectrophotometrically analysis BioPhotometer Plus, Eppendorf, Manchester, United Kingdom, nm wavelength.

Red blood cells were treated with D-Gal, with or without pre-incubation with peel or juice extract. As the positive control, cells were incubated with H 2 O 2. Superoxide dismutase activity was determined by reading the absorbance at nm wavelength Fluostar Omega, BMG Labtech, Ortenberg, Germany after subtracting the background.

Red blood cells were treated with D-Gal with or without pre-incubation with peel or juice extract. As the positive control, cells were treated with H 2 O 2. Catalase activity was determined by reading the absorbance at nm wavelength Fluostar Omega, BMG Labtech, Ortenberg, Germany after subtracting the background.

RBCs were left untreated or treated with D-Gal, with or without pre-incubation with peel or juice extract. The fluorescence intensity is proportional to the G6PDH activity in the samples. Determination of the reaction rate was performed by a plate spectrophotometer Onda Spectrophotometer, UV to monitor NAPDH rate of production, such molecule absorbs light at nm, over a 30 min time course.

GSH levels were quantified according to Teti and collaborators Teti et al, Sample absorbance was measured at nm Onda spectrophotometer, UV All data are expressed as arithmetic means ± standard error of the mean.

GraphPad Prism version 9. Table 1 reports qualitative and quantitative features of bioactive molecules deriving from bergamot juice and peel samples.

As seen in Table 1 , the two sample sources showed the same qualitative profile. TABLE 1. Each sample was analyzed in triplicate. On the other hand, juice and peels differed from a quantitative point of view.

Peel sample was richer in bioactive molecules 16, Neohesperidin was the most abundant bioactive compound in these samples. As expected, incubation of RBCs with mM D-Gal for 24 h at 25°C led to a substantial increase in ROS and TBARS levels as well as a reduction in -SH group content compared to untreated RBCs Supplementary Figure S3 , which denotes induction of oxidative stress and is consistent our former findings Remigante et al, c.

Increasing concentration and pre-incubation times revealed a clear antioxidant effect of the peel and juice extracts, as denoted by a significant reduction in ROS and TBARS levels, as well as increase in total SH group content compared to D-Gal treated RBCs Supplementary Figures S3D—I.

Figure 1A displays the intracellular ROS levels at 0 and 24 h after exposure to D-Gal. As seen, mM D-Gal treated samples showed a significant increase of ROS levels compared to left untreated samples.

As expected, ROS levels of RBCs treated with 20 mM H 2 O 2 for 30 min were significantly higher with respect to those of RBCs left untreated control. In addition, peel or juice extract alone did not significantly alter the intracellular ROS levels data not shown.

FIGURE 1. Antioxidant capacity estimation of peel and juice extract. human RBCs have been left untreated or exposed to 20 mM H 2 O 2 or 2 mM NEM for 1 h or mM D-Gal for 24 h at 25°C with or without pre-exposure to peel or juice extract for 15 min at 37°C. Thiobarbituric-acid-reactive substances TBARS levels measured in RBCs are reported in Figure 1B.

As expected, TBARS levels after treatment with 20 mM H 2 O 2 for 1 h were significantly higher than those detected in control left untreated RBCs.

Similarly, after 24 h treatment with mM D-Gal, TBARS levels were significantly increased with respect to control.

Of note, peel or juice extract alone did not significantly alter TBARS levels Supplementary Figures S1D, S2D. As expected, treatment with NEM led to a significant reduction in the content of the sulfhydryl groups compared to untreated RBCs control. Sulfhydryl groups in - RBCs treated with mM D -Gal were also significantly lower than control.

Additionally, peel or juice extract alone did not significantly alter the total content of the sulfhydryl groups Supplementary Figures S1G, S2G. The levels of band 3 expression were significantly decreased in human RBCs incubated with mM D-Gal for 24 h with respect to control Figure 2A.

Moreover, Band 3 distribution was assessed by immunofluorescence technique. In particular, band 3 was mainly clustered arrows in leptocytes after mM D-Gal exposure, with respect to untreated RBCs Figure 2B.

Of note, band 3 expression was altered neither by peel nor by juice extracts given alone data not shown. FIGURE 2. Flow cytometry immunofluorescence of band 3 expression. A Histograms report median values of fluorescence intensity. Samples were observed with a × objective.

Note the significant morphological changes in mM D-Gal arrows. The transport rate constant in RBCs treated with mM d -Gal 0. FIGURE 3. Red blood cells were also exposed to a specific inhibitor of band 3 10 µM DIDS. TABLE 2. Data are presented as means ± S.

from n separate experiments. In Figure 4 , A1c levels measured in diabetes patients -used as the positive control-are also reported Chandalia and Krishnaswamy, As expected, a significant increase in the A1c levels were measured in RBCs from diabetic patients. FIGURE 4. In RBCs exposed to D-Gal, the SOD activity was found significantly increased compared to the untreated cells.

Conversely, pre-incubation with peel or juice extract resulted in a significant recovery of SOD activity with respect to D-Gal-treated cells. As expected, SOD activity in RBCs treated with 20 mM H2O2 for 30 min at 25°C as the positive control was significantly higher than that of control RBCs.

Of note, peel and juice extracts did not significantly alter SOD activity when given alone data not shown. FIGURE 5. Effects of D-Gal mM exposure with or without pre-treatment with peel or juice extract for 15 min in RBCs.

A SOD activity, B CAT activity, and C G6PDH activity. D-Gal treatment caused an increased CAT activity compared to control cells, which was consistent with an elevated oxidative stress Figure 5B.

Exposure to 20 mM H 2 O 2 for 30 min 25°C has been considered as the positive control. As expected, CAT activity in RBCs treated with H 2 O 2 was significantly higher than those of control RBCs.

Moreover, the extracts of peel and juice alone did not significantly alter CAT activity data not shown. G6PDH activity was severely stimulated by an increased oxidative stress in the D-Gal-treated cells compared to control RBCs. On the contrary, in cells pre-exposed to peel or juice extract, G6PDH activity was brought back to the control levels.

As expected, G6PDH activity of RBCs treated with G6PDH positive control supplied by the manufacturer for 30 min at 25°C was significantly higher than that of RBCs left untreated.

In addition, both peel and juice extracts did not significantly alter G6PDH activity data not shown. However, pre-incubation with peel and juice extract restored GSH levels.

FIGURE 6. GSH, reduced glutathione; GSSG, oxidized glutathione. Recently, an increasing body of evidence has supported the hypothesis that natural molecules -referred to as antioxidants-may have a protective role in retarding or reversing the course of age-related diseases Tan et al, ; Singh et al, ; Lu et al, ; Remigante et al, b ; Cui et al, These compounds are able to compete with substrates which are sensitive to oxidation, thus inhibiting or delaying the reactions between reactive species and biological macromolecules Pisoschi and Pop, Even though reactive species are involved in several biological processes, their over-production can lead to cell damage and consequently, development of chronic diseases Costa et al, ; Luo et al, ; Bertelli et al, ; Remigante et al, b ; Forman and Zhang, ; Zuccolini et al, Herein, the composition in bioactive molecules of bergamot peel and juice Table 1 and their effects on oxidative-stress induced aging were described in a cell-based model represented by human RBCs subjected to prolonged 24 h exposure to mM D-Gal.

This cell-based model, which was validated in our former studies Remigante et al, c ; Remigante et al, d ; Spinelli et al, , recapitulates the cellular and molecular mechanisms of natural aging, i.

Though various investigations described the numerous activities of bioactive compounds of bergamot extracts, their effects on aging RBCs have not yet been fully analysed. This is not surprising, given that various antioxidants may act as pro-oxidants depending on their concentration Osseni et al, ; Sahebkar, ; Giordano et al, These and our findings point to the importance of carefully assessing concentration and incubation time when novel potential antioxidant compounds are tested in cell-based assays.

This latter acts as a catalyst in redox reactions and lipid peroxidation, resulting in TBARS production as the final product Pandey and Rizvi, Since ROS generated during cellular metabolism cause the oxidation of biological macromolecules, the effect of peel and juice extract on the intracellular ROS levels has been evaluated as a first step of the investigation.

To better elucidate the process of oxidation of plasma membrane macromolecules, the estimation of both TBARS level and sulfhydryl group content of total proteins, which in RBCs mainly belong to band 3 protein Rao and Reithmeier, , have been investigated.

These findings denote that peel or juice extract could effectively protect both the lipid and protein component of the RBC plasma membrane from oxidation. Data related to oxidative stress assessment are in line with what previously showed by other researchers and suggest that polyphenols and phytochemicals could play scavenger activity by directly neutralizing reactive species and free radicals and avoid their detrimental impact on biological macromolecules Zhao et al, ; Kelen and Tepe, ; Luo et al, ; Kruk et al, One of the most interesting and still unknown implications of oxidative stress-related aging is its impact on plasma membrane transport systems.

The structure of this protein consists of two rather different domains of a similar size Arakawa et al, The evidence that oxidative stress provokes functional modifications of band 3 in human RBCs has been provided also in other cell-based models of oxidative stress.

Thus, a possible a two-faced effect on the velocity of anion exchange could depend on specific cell component lipids, proteins, as well as enzymes affected by both the stressors and the related pathways.

Based on data hitherto obtained, we can point out that both extracts show a similar protective effect on anionic exchange and could, therefore, act a key role in counteracting oxidative stress—induced functional changes in human RBCs. The binding sites for cytoskeletal and cytoplasmic proteins, including haemoglobin, are located on the N-terminal cytoplasmic domain of band 3 Anong et al, ; Wu et al, Haemoglobin glycation represents a case of non-enzymatic protein glycation associated with production of AGEs Luevano-Contreras and Chapman-Novakofski, To better explore the molecular interaction between band 3 and haemoglobin, levels of both proteins were evaluated.

The data obtained show that mM D-Gal treatment caused both a loss and a redistribution of band 3, most probably due to the shedding of protein-containing vesicles Kuo et al, Figures 2A, B. The shedding of membrane area is a critical point for cell fate, since a reduced surface-to-volume ratio is thought to correlate with the early phagocytosis of aged RBCs Kuo et al, Consequently to membrane shedding, the cytoskeleton reduces to a 3- to 5-fold smaller area Li and Lykotrafitis, The proteins affected are mainly band 3, ankyrin, spectrin, and occasionally protein 4.

These proteins are all part of one of the complexes by which the cytoskeleton is anchored to the lipid bilayer Gardel et al, ; Anong et al, ; Vallese et al, Presumably, damage of these proteins is responsible for the impaired cellular deformability associated with oxidative stress Mohanty et al, A decrease in RBC deformability leads to impaired microcirculation and tissue oxygenation Schwartz et al, ; Silva-Herdade et al, For example, Spinelli and co-authors have shown that both band 3 phosphorylation and rearrangements in cytoskeleton proteins can lead to cell deformation and structural alteration of human RBCs during the aging processes Spinelli et al, As mentioned above, haemoglobin is anchored to band 3 cytoplasmic domain.

In this regard, our findings indicated that exposure of RBCs to mM D -Gal for 24 h increased the content of A1c levels Figure 4 , contributing to both biochemical and structural changes, including clustering of band 3 regions.

At a more advanced stage of aging, such clusters could represent a recognition site for antibodies directed against aged RBCs, thus triggering the early removal of RBCs from the blood circulation Briglia et al, Collectively, these findings Figures 2 , 4 suggest that the active biomolecules of the bergamot juice or peel extract might efficiently prevent RBCs membrane shedding, formation of glycated haemoglobin, and RBCs structural instability, all of which are hallmarks of aging.

At last, we investigated the activity of endogenous antioxidant enzymes CAT and SOD Figures 5A, B. These antioxidant enzymes own outstanding free radical scavenging capacities and play vital roles in human RBCs Ulanczyk et al, The SOD and CAT activity in RBCs incubated with mM D-Gal was much higher than in control cells, which could reflect the activation of the endogenous antioxidant defense system to suppress the formation of free radicals Figure 1A.

Nevertheless, the increase in SOD and CAT activity failed to compensate for the increase in free radicals Figure 1A , as also demonstrated by the increase in lipid peroxidation levels as well as total protein oxidation Figures 1B, C. Upregulation in CAT and SOD activity with concomitant substantial elevation of oxidative stress markers might reflect exhaustion of the endogenous antioxidant system.

These biochemical changes may provoke injury to the membrane proteins and lipid structure. As a result, the membrane mechanical properties could be modified, resulting in deformability and fluidity reduction or altered permeability of the phospholipid bilayer, which, in turn, reduce the ability of the membrane to withstand osmotic changes Spinelli et al, In this context, pre-exposure of RBCs to peel and juice extracts could significantly prevent the upregulation in both SOD and CAT activity observed in D-Gal-treated cells Figures 5A, B.

These findings denote that the active biomolecules of the bergamot juice or peel extract might act synergistically with endogenous antioxidant system to counteract oxidative stress in RBCs and preserve cell integrity.

RBCs are unable to generate ATP using molecular oxygen because of lack of mitochondria. Glycolysis is the only source of ATP generation in mature RBCs. Glucosephosphate dehydrogenase G6PDH is a crucial enzyme of this latter pathway, which produces reduced NADPH and represents the most important defense mechanism for RBCs in case of excessive oxidant generation, or of ineffective antioxidant defense Maha, In these conditions, glycolysis and its key enzyme GAPDH are inhibited Reisz et al, In addition to its importance in the RBC as a major metabolic determinant during oxidative stress, G6PDH activity is also employed as an indication of cell aging Subasinghe and Spence, The G6PDH activity was severely stimulated - presumably by an increased oxidative stress - in D-Gal-treated cells compared to control RBC cells Figure 5C , which was consistent with inhibition of glycolysis and activation of the PPP shunt.

In addition, as already mentioned, the underlying mechanisms of aging can severely alter enzyme activities, including glutathione Maher, Glutathione is an important endogenous non-enzymatic antioxidant that neutralizes ROS production Ahmad and Mahmood, Its depletion makes cells more prone to oxidative damage.

However, the pre-incubation of RBCs with peel and juice extract partially restored the redox balance Figure 6.

In summary, our findings suggest that the active biomolecules of the bergamot juice or peel extract might prevent metabolic alterations in RBCs exposed to oxidative stress.

Here we characterize the precise composition in bioactive compounds of the bergamot Citrus bergamia, Femminello cultivar peel and juice extracts and we assess their protective effect in an oxidative stress-related model of cellular aging in RBCs.

The data presented here indicate, for the first time, that polyphenol-rich extracts of peel and juice from bergamot fruit may act on oxidative stress-induced alterations of the lipid and protein cellular components, including the endogenous antioxidant system as well as proteins with ion transport activity and enzymatic metabolic activity, thus protecting the structural and functional integrity of human RBCs.

This study identifies bergamot as an antioxidant functional food and further suggests that diet supplementation with bergamot or its derivatives might contribute to the prevention or attenuation of pathophysiological events linked to RBCs dysfunction during aging.

The raw data supporting the conclusion of this article will be made available by the authors, without undue reservation.

The studies involving human participants were reviewed and approved by the study was conducted in accordance with the Declaration of Helsinki and approved by the Institutional Review Board or Ethics Committee of University of Messina prot.

AR and RM conceived and designed the research; SS, MR, GC, ES, LG, DC, GF, and PD performed the experiments and analyzed the data; AR and RM interpreted the results of the experiments; SS and AR prepared the figures; AR drafted the manuscript; AM, AR, and SD edited and revised the manuscript.

All authors contributed to the article and approved the submitted version. The authors thank 1 Azienda Agricola Cilea Rosaria for kindly providing us bergamot fruits, 2 Shimadzu and Merck Life Science corporations for the continuous support, 3 Professor Caterina Faggio from University of Messina for helpful discussion, and iv Dr.

The best studied use of vitamin C for photoprotection is as a topical agent. A Bayesian meta-analysis of 31 clinical trials involving Chinese and Caucasian subjects found that topical vitamin C was effective in reducing UV light-induced pigmentation in a dose dependent manner [21]. The clinical trials revealed that vitamin C was effective in preventing pigment formation, rather than removing existing melanin which is coherent with its antioxidant properties.

Even though oral dosing of vitamin C was not the focus, this meta-analysis does demonstrate the ability of vitamin C to protect against photoaging of the skin. Furthermore, increased dietary intake of vitamin C has been shown to result in significant rises in both plasma and skin vitamin C content [39].

Additional analysis revealed that maximum radical scavenging activity was reached within two weeks [40]. In healthy volunteers, ingestion of vitamin C, combined with vitamin E, for 8 days increased the minimum erythemal dose MED of UV light from 80 to The increased threshold for UV energy required to initiate erythema demonstrates that the antioxidant vitamins inhibited the initiation of ROS production in the skin.

The oral intake of vitamins C and E for three months significantly reduced the sunburn reaction to UVB irradiation in healthy volunteers.

Additionally, UV-induced thymine dimers DNA damage in the skin were decreased following vitamin supplementation [42]. Grapes Vitis vinifera are one of the most widely consumed fruits in the world and have a very long history of human food use. During the processing of grapes for juice and wine, the seeds are discarded.

An extract produced from the seeds is rich in polyphenolic compounds, especially proanthocyanidins condensed tannins.

In addition to cyanidinglucoside, the extract includes gallic, protocatechuic, chlorogenic, caftaric, caffeic and trans-coutaric acids as well as ellagic acid, quercetin, kaempferol, luteolin and dihydrofisetin derivatives [43].

The oligomeric and polymeric procyanidins in grape seed are composed of catechins, procyanidin dimers, epicatechin gallate, procyanidin dimer gallates, procyanidin dimer digallates, procyanidin trimers, and procyanidin trimer gallates [44].

Grape seed extract has notable cardiovascular system benefits. Grape seed extract may also provide other positive effects on human health through its anti-inflammatory, immune modulating and antimicrobial properties, to name just a few [47].

But the most salient feature of grape seed extract is its powerful antioxidant action [48]. Its exceptional antioxidant activity is a major factor in the anti-photoaging properties of grape seed extract.

Human keratinocytes were cultured with or without grape seed extract for 30 minutes prior to UVB irradiation. Cells that were pre-treated with grape seed extract experienced increased cell viability, lower lipid peroxide levels or less oxidative damage and fewer DNA photolesions [49].

In another study, grape seed extract also decreased the level of ROS in UVB-irradiated human keratinocytes in vitro. Further, NF-kB p65 protein levels, a marker of inflammatory signaling, increased in untreated cells following UVB exposure.

But NF-kBp65 remained at pre-UVB exposure levels in cells cultured with grape seed extract [50]. DNA repair was significantly increased in xeroderma pigmentosum complementation group A XPA -proficient human fibroblasts that were cultured with grape seed proanthocyanidins GSPs for 3 hours while simultaneously being exposed to UVB light.

GSPs further enhanced nuclear translocation of the XPA protein and increased its interactions with ERCC1, a DNA excision repair protein [51]. These in vitro experiments demonstrate that GSP is not only capable of curbing the initiation stage of photodamage, by inhibiting ROS formation and inflammation, but may also amplify DNA repair mechanisms in skin cells.

Feeding of GSPs inhibited of UV-induced cyclooxygenase-2 COX-2 expression, prostaglandin synthesis, leukocyte infiltration and myeloperoxidase induction in the skin of SKH-1 hairless mice.

Pro-inflammatory cytokines TNF- α , interleukin 1 β and IL-6 were also lower in the skin of GSP fed mice than in control mice [52].

Chronic inflammation involves elevated production of proteolytic enzymes which then degrade the extracellular matrix of the dermis [53]. As such, the anti-inflammatory action of GSPs will reduce the UV-induced degradation in skin quality that occurs with sun exposure.

Orally administered grape seed polyphenols decreased macrophage infiltration into and elastin degradation of aorta tissue as well as reduced expression of MMP-2 and MMP-9 [54].

The overall effect was to prevent excessive dilation of the aorta following repeated exposure to elastase. While this experiment did not directly involve UV irradiation of the skin, similar pathological processes were involved.

And these results provide additional evidence of the ability of phytochemicals in grape seed extract to protect the structure and function of the extracellular matrix. The addition of GSP to the diet of SKH-1 hairless mice for two weeks reduced tumor incidence, multiplicity and size after 30 weeks of UVB exposure to the skin.

Biochemical analysis revealed significant reduction in lipid peroxidation, thus suggesting that the antioxidant activity of GSP is a major photoprotective mechanism [55]. Further, GSP ingestion maintained glutathione peroxidase, catalase, and glutathione levels while reducing hydrogen peroxide, lipid peroxidation, protein oxidation, and nitric oxide concentrations in the skin of UVB exposed mice.

GSP also inhibited mitogen activated protein kinases MAK and nuclear factor- κβ NF- κβ signaling [56]. Additional research investigated the ability of GSP to prevent UVB-induced immunosuppression as another mechanism of protection against the development of skin cancer.

In these studies, dietary GSPs mitigated UVB-induced suppression of dermal immune responses and increased IL production. Intraperitoneal injection of a neutralizing anti-IL antibody abolished the immune protective impact of GSP [57]. From these results, it appears that antioxidant, anti-inflammation and immunomodulating activities all have some role in the photoprotective potential of grape seed extract in SKH-1 hairless mice.

Another line of anti-photoaging evidence involves melanogenesis. Feeding of grape seed extract to guinea pigs for 8 weeks resulted in less UV-induced skin pigmentation than that which occurred in control animals. This anti-melanogenic effect appears to involve antioxidant action as there was a decrease in ROS-related proliferation of melanocytes.

Japanese melasma hypomelanosis patients ingested proanthocyanidin-rich grape seed extract for 11 months, divided into two phases with a one-month break in treatment after the first 6 months.

Sun exposure is a major factor in melasma. UV light is the primary causative agent that combines with other underlying factors to produce large areas of hyperpigmentation on the face.

Since melasma is an excessive response to UV light exposure, ROS-mediated symptoms are reduced by dietary grape seed extract due to increased antioxidant capacity of the skin.

A 5-day clinical trial revealed that daily ingestion of grape seed extract increases serum total antioxidant activity. No such increase was observed in the placebo group [61]. Again, photo damage is initiated by the production of ROS generation. Subsequent propagation of oxidative damage is attenuated by antioxidant action, and increased antioxidant activity in the serum directly translates to improved skin quality [62].

A few other clinical trials have been completed in which participants ingested dietary supplements containing grape seed extract as low as In these studies, ingestion of supplements containing grape seed extract significantly increased collagen and elastic fiber density both on the face and arm as well as skin luminosity by Significant improvements in erythema, skin hydration, skin radiance, overall appearance including fine lines and wrinkles and dermal ultrasound density were also evident.

Mottled pigmentation of the skin was also significantly reduced [63] [64] [65] [66]. Citrus species are some of the most widely cultivated fruits in the world. Citrus fruit, juices, and processed compounds are major sources of nutrients in the human diet.

Citrus is a well-known source of bioactive compounds, with flavonoids being major components [67]. Flavonoids are a large group of polyphenolic secondary metabolites widely occurring in plants.

Flavonoids exhibit a wide range of biological activities. Hesperidin is reported to be the major flavonoid in orange and lemon [68] [71]. The bioflavonoids naringin, isonaringin and didymin are also found in different parts of various citrus fruits [72] [73]. Naringin and isonaringin were found to be the main flavonoid in fresh, oven-dried, and freeze-dried peels of grapefruit, an important cultivar of the Citrus genus [72].

A wide range of biological and pharmacological activities have been reported for hesperidin [68] [74]. Citrus fruits and their flavonoids have potent anti-aging and photoprotective effects on the skin and could serve as powerful bioactive skincare agents [75]. The effects of hesperidin on UVA-induced skin oxidative stress and inflammation, as well as the underlying mechanisms of action, were evaluated in human keratinocytes [76].

The results of this study indicated that hesperidin directly protects cells from UVA-induced cell damage. These data indicate that hesperidin protects keratinocytes from both UVA- and UVB-induced damage and may involve anti-oxidative and anti-inflammatory activities.

Consequently, the authors of this study suggested that hesperidin might be a useful sunscreen agent. Hesperidin was also investigated for its ability to prevent apoptosis due to UVB-generated oxidative cellular stress.

Furthermore, hesperidin scavenged the 2,2-Diphenylpicrylhydrazyl DPPH radical in a dose-dependent manner [77]. In summary, the results indicate that hesperidin may scavenge ROS, absorb UVB irradiation and regulate apoptotic proteins, thus protecting human keratinocytes against UV damage.

Additionally, we observed in our laboratory that a preparation of hesperidin-rich Citrus aurantium extract, combined with vitamin C and grape seed extract Vináli , quite rapidly and thoroughly scavenged the DPPH radical. The immune-modulatory activity of hesperidin was observed in multiple studies.

The same dose of hesperidin was also found to increase the development of immunological memory in the cellular immune response. Treatment of UVA-irradiated human fibroblasts with 0.

Naringenin and naringin, well-known antioxidants, have been shown to extend the life span and health of the Caenorhabditis elegans under normal conditions as well as during UVA-induced stress. This may be due to regulation of two stress-responsive genes skn-1 and sir Further, a synergistic anti-aging effect was observed when a combination of these compounds, in equal ratios by weight, were tested.

Jung et al. studied on the effect of naringenin on UVB-induced matrix MMP-1 expression and its direct target [82]. Like all-trans retinoic acid, a US FDA approved anti-wrinkle drug, naringenin was found to significantly inhibit UVB-induced MMP-1 expression.

This effect was further confirmed by an immunofluorescence experiment within the study. Naringin decreases oxidative UVB radiation damage in NIH-3T3 cells and the associated inflammatory response by modulating PPAR- γ expression.

As such, it can effectively prevent UVB-mediated DNA damage, photoaging, and apoptosis [83]. In another in vitro assay, fresh and oven-dried grapefruit peel extracts displayed strong cytoprotective properties in SH-SY5Y neuroblastoma cell cultures at concentrations ranging from 0.

Didymin from citrus fruit was reported to reduce the effects of UV stress on nematodes C. elegans by decreasing ROS levels and increasing superoxide dismutase SOD activity [84]. ROS levels are correlated with age-related phenotype.

UV-induced bodily damage causes an increase in ROS levels. But didymin, at 0. Kim et al. The results of this study indicate that HMF inhibits collagenase activity, increases type I procollagen content in UV-induced HDFn cells and suppresses MMP-1 expression.

In addition, HMF was found to affect the MAPK signaling pathway. These results suggest that HMF may possess photoprotective properties. Lee et al.

investigated the anti-photoaging potential of hesperidin on dorsal skin in hairless mice [85]. Every day, six-week-old hairless male mice were administered 0. These animals were then exposed to UV light, and changes in skin wrinkle length and depth were measured. In the hesperidin-treated group, the average length and depth of wrinkles were significantly less than in the control group.

Further, hesperidin may reduce expression of MMP-9 and pro-inflammatory cytokines. Hesperidin was reported to decrease tryptophan intensity and interact with collagenase. It may inhibit metalloproteinases collagenase, elastases, and hyaluronidases by chelating their metal ions.

Further, a face cream containing hesperidin nanoparticles - nm may reduce black circles in the under eye region. Additionally, daily topical application of a hesperidin nanoemulsion led to a significant skin whitening, reduction in trans-epidermal water loss, and inhibition of skin irritation after exposure to UV rays [86].

Immature Citrus unshiu powder ICP contains high concentrations of flavonoids such as hesperidin and narirutin. Ingestion of ICP increased epidermal cell growth, suppressed epidermal cell mortality and prevented basement membrane destruction in the skin of UVB irradiated hairless mice [87].

ICP also improved skin hydration and decreased transepidermal water loss. An SKH-1 hairless mouse model was also employed to study the anti-photoaging of naringenin in vivo [82]. In this model, the dorsal skin was exposed to UVB three times a week with the irradiation dose being increased weekly from 1 MED to 4 MED.

The 4 MED UV dose was maintained until 15 weeks, after which skin surface impression were obtained and analyzed. During this study, the naringenin-treated group experienced significantly less MMP expression. This inhibitory effect occurred mainly through the blockage of ERK2 kinase activity.

Martinez et al. applied a topical naringenin containing formulation to hairless mice to examine its potential for reducing UVB irradiation-induced skin inflammation and oxidative damage [88].

The topical application of the naringenin formulation protected mouse skin by inhibiting edema and cytokine production TNF- α , IL-1 β , IL-6, and IL In an experiment performed by Tirkey et al.

Hesperidin fed to mice also reduced superoxide generation in electron transfer and concerted proton transfer reactions [90]. A recent review of computational and experimental studies revealed that hesperidin, together with other flavonoids such as naringin, may outperform other drugs in COVID prophylaxis and treatment clinical trials [91].

Hesperidin appears to have high binding affinity for the main cellular receptors of SARS-CoV-2 and possibly dampens excessive proinflammatory responses by the immune system. Hesperidin strongly inhibited rotavirus infectivity.

A combination of citrus bioflavonoids and rosemary extract was evaluated for its photoprotective potential using human HaCaT keratinocytes as well as human volunteers. The combination of these two ingredients increased survival rates of HaCaT cells, following UVB irradiation, more than the individual extracts alone, suggesting potential synergic effects.

The combination also decreased UVB-induced intracellular ROS and prevented DNA damage. It also decreased ex-vivo chromosomal aberrations in X-irradiated human lymphocytes isolated from the volunteers who had ingested it. The authors of this study suggested that ingestion of the combined extracts may be an alternative treatment to topical sunscreen application [92].

Exposure to UV light leads to skin inflammation. A systematic review and meta-analysis of randomized controlled clinical trials RCTs was conducted by Lorzadeh et al.

to evaluate the effect of hesperidin supplementation on inflammatory markers [93]. The authors used a random-effects model to examine the differences in inflammatory markers between hesperidin supplementation and a control group. With participants included in the study, the analysis showed that hesperidin supplementation significantly decreased vascular cell adhesion molecule 1 VCAM-1 levels.

As VCAM-1 is a key cell adhesion molecule involved in inflammation, the results confirm the clinical anti-inflammatory properties of hesperidin supplementation. A placebo-controlled, randomized and double-blinded clinical trial was performed to evaluate the impact of hesperidin supplementation on the cognitive function of 37 senior healthy adults 60 - 81 years of age [94].

In this trial, participants were assigned to drink of one of two dietary interventions twice per day.

The study also found that the chronic consumption of hesperidin-rich juice significantly decreases diastolic blood pressure.

This suggests that hesperidin-rich dietary interventions can prevent cognitive decline in neurodegenerative patients by increasing cerebral blood flow.

Dietary antioxidants have the potential to provide protection against skin photoaging. Results of in vitro , in vivo and human studies involving vitamin C, grape seed extract, and citrus bioflavonoids support the concept that antioxidant supplementation quenches UV-induced ROS generation in the skin.

Further, these substances also reduce inflammation and expression of proteolytic enzyme which are involved in the premature aging of the skin. Each of these well-known antioxidants exhibits multiple other health benefits to the human body.

Therefore, there is ample reason to recommend regular ingestion of these natural antioxidants as a means of maintaining skin health. The authors declare no conflicts of interest regarding the publication of this paper.

Manifestations, Prevention, and Treatment. Dermatologic Clinics, 4, and Maibach, H. International Journal of Cosmetic Science, 30, x [ 3 ] Meinhardt, M. anders, A. and Tronnier, H. Journal of Biomedical Optics, 14, Article ID: Journal of Investigative Dermatology, , E2-E6.

and Fonferko, E. The Elastic Fiber Network. Journal of Investigative Dermatology, 78, ep [ 6 ] Rittie, L. and Fisher, G. Cold Spring Harbor Perspectives in Medicine, 5, a a [ 7 ] Warren, R.

and Ridder, G. Journal of the American Academy of Dermatology, 25, and Uitto, J. Photoaging: A Comparative Histopathological, Immunohistochemical, and Ultrastructural Study of Skin. Experimental Dermatology, 11, x [ 9 ] Hanson, K.

and Simon, J. Proceedings of the National Academy of Sciences, 95, and Scharffetter-Kochanek, K. Journal of Biological Chemistry, , and Bruijnzeel, P. Journal of Investigative Dermatology Symposium Proceedings, 14, and Vershoore, M. These compounds include a wide array of flavonoids and have been found to offer a number of health benefits when taken as a supplement.

Citrus supports healthy cholesterol levels and supports blood cell health and function. When used as a supplement for supporting healthy cholesterol, mg of Bergamont Extract should be taken per day until cholesterol reaches healthy levels.

After this, a mg maintenance dose is recommended and can be taken indefinitely. It is not recommended to take more than mg of Citrus Bergamont Extract per day.

We prioritize your well-being and are committed to delivering exceptional quality in every product we offer. Your health is our priority. For any questions or assistance, contact our customer service team.

Trust in the quality of Double Wood Supplements. Citrus Bergamot Extract Triple Pack. At Double Wood Supplements, we invest significantly in research and development to achieve the highest standards of product quality. We utilize third-party testing to assess the purity and quality of our supplements.

Moreover, we proudly provide certificates of analysis for every product, openly sharing the results with our customers, a practice uncommon in the industry.

Introduction

Changes in intestinal microbiome phylum and genus families at 19 and 70 weeks old in P1 mice drinking water or 0. Genus-level differences in the microbiome among the 3 groups were evaluated. An increase in Lactobacillus strains associated with aging has been reported in human microbiota Eriocitrin is metabolized by intestinal bacteria, after which the metabolites are absorbed 24 , increasing anti-oxidative activities in the plasma 24 and decreasing oxidative stress We predicted that the anti-aging effects of LPP are developed through the intestinal microbiome both directly and indirectly.

We previously reported that the intestinal microbiota varies between breeder companies because of their different breeding environments, even in the same strain of mice In this aging study, we used the same breeding chamber and same type of cages.

Therefore, the changes in the microbiota appear to be related to aging rather than the breeding environment. Arumugam et al. Remarkable changes in key microbiota for the human enterotype 35 , 36 were observed by the LPP intake and aging in this study.

Our results suggested that lifelong intake of LPP has anti-aging effects not only on the host health status but also on the intestinal environment. Recently, Henning et al. Additional studies are needed to clarify whether intestinal or phenotypic changes occur at first during the intake of polyphenols.

Additionally, possible aging-related changes in the intestinal microbiomes, such as for the genus Lactobacillus , were restricted by LPP intake. These results suggest that LPP has anti-aging effects not only on host health but also on the intestinal environment.

Evaluating lifelong intake of food is important for detecting the effects on human and animals because they are likely to consume habitual diets throughout their lifespan. Food habits may exert a large influence on the host. Lemon polyphenols LPP from lemon peel were obtained as described in a previous report We freshly prepared 0.

Thirty-six SAMP1 male mice aged 5 weeks and 18 SAMR1 Japan SLC, Inc. The floor of the cage was covered with sliced paper, Palmas μ® Material Research Center, Tokyo, Japan , which was changed every week. SAMR1 mice were group-housed six mice per cage with free access to tap water.

All mice were provided ad libitum access to standard chow CRF-1; Charles River Laboratories, Yokohama, Japan. We used the same breeding chamber EBAC-L ® , CLEA Japan, Inc. All experiments were approved by the Institutional Animal Care and Use Committee of SAPPORO BREWERIES LTD.

permit number following the Guidelines for the Proper Conduct of Animal Experiments on the Science Council of Japan. All experimental protocols and animal procedures were conducted in accordance with the approved guidelines. Food consumption and liquid consumption per cage were recorded every week and three times every week, respectively.

The body weight of each mouse was examined every week. During the lifespan of all mice, we examined the changes in grading scores, such as those for skin and hair conditions glossiness, hair coarseness, and hair loss , ulcers, eyes cataract, periophthalmic lesions, opacity of cornea, and ulcer of cornea , and skeleton spinal curvature nearly every month from 6 weeks to 88 weeks old, as reported previously The novel ORT and OLT for spatial cognition are non-invasive and conducted under conditions similar to those used for human cognitive assessment We measured cognitive functions by ORT and OLT as previously reported For OLT objects, apple-shaped wooden blocks without colouring mm width and mm height of main body and mm height of stem end were used.

The ORT experiments were conducted at 8, 22, 34, 50, 66, and 79 weeks old and the OLT experiments were conducted at 13, 14, 23, 35, 51, 67, and 80 weeks old to evaluate cognitive function.

Differences between recognition indices of left and right or novel location objects were assessed by using the unpaired t -test for ORT OLT in each phase. Illumination was provided at 25 lux. A black patch was attached to the back of each mouse to enable tracking in the ORT box with a white-coloured floor.

Bacterial DNA was isolated as described by Matsuki et al. Primers for amplification of the V1 and V2 regions of the 16S rRNA gene reported by Kim et al.

PCR was performed in a μL reaction volume. Each reaction mixture contained After each reaction, the mixture was purified using a PureLink Quick PCR Purification Kit Invitrogen, Carlsbad, CA, USA ; the concentration of each purified sample was measured using a Qubit 2. Purified samples were mixed at equal concentrations.

Emulsion PCR and sequencing were carried out by Ion PGM sequencing systems Life Technologies. The values are presented in the relative abundance of microbiota. SPSS software Between-group comparisons between bacterial species were performed by unpaired t -tests. Kajimoto, O.

The internet investigation about the attenuation of fatigue feeling by taking a drink containing lemon citric acid. in Japanese. Google Scholar. Miyake, Y. et al. Lipid-lowering effect of eriocitrin, the main flavonoid in lemon fruit, in rats on a high-fat and high-cholesterol diet.

Food Sci. Article CAS Google Scholar. Hiramitsu, M. Eriocitrin ameliorates diet-induced hepatic steatosis with activation of mitochondrial biogenesis. Article Google Scholar. Isolation of antioxidative phenolic glucosides from lemon juice and their suppressive effect on the expression of blood adhesion molecules.

Unno, K. Daily consumption of green tea catechin delays memory regression in aged mice. Biogerontology 8 , 89—95 Porquet, D. Age Dordr. Takeda, T. A new murine model of accelerated senescence. Ageing Dev. Takahashi, R. Anti-aging studies on the senescence accelerated mouse SAM strains.

Yakugaku Zasshi , 11—18 Aoyama, Y. Impaired motor function in senescence-accelerated mouse prone 1 SAMP1. Brain Res. Yan, J. Reduced coenzyme Q10 supplementation decelerates senescence in SAMP1 mice.

Turnbaugh, P. A core gut microbiome in obese and lean twins. Nature , — Article ADS CAS Google Scholar. Lewis, J. Cell Host Microbe 18 , — Gut microbiota and aging. Science , — Article ADS Google Scholar.

An obesity-associated gut microbiome with increased capacity for energy harvest. Mitsuoka, T. Intestinal flora and aging.

Odamaki, T. Age-related changes in gut microbiota composition from newborn to centenarian: a cross-sectional study.

BMC Microbiol. Characteristics of antioxidative flavonoid glycosides in lemon fruit. Folin-Ciocalteu method ISO ; Determination of substances characteristic of green and black tea—Part 1: Content of total polyphenols in tea—Colorimetric method using Folin-Ciocalteu reagent.

html Okawa, M. DPPH 1,1-diphenylpicrylhydrazyl radical scavenging activity of flavonoids obtained from some medicinal plants. Biol Pharm Bull. Wu, X. Lipophilic and hydrophilic antioxidant capacities of common foods in the United States. J Agric Food Chem. Protective effects of lemon flavonoids on oxidative stress in diabetic rats.

Editor-inchief The senescence-accelerated mouse SAM achievements and future directions, ELSEVIER Boldyrev, A. Antioxidant systems in tissues of senescence accelerated mice. Biochemistry Mosc 66 , — Identification and antioxidant activity of flavonoid metabolites in plasma and urine of eriocitrin-treated rats.

Food Chem. Beyer, I. Chronic low-grade inflammation and age-related sarcopenia. Care 15 , 12—22 Meng, S. Oxidative stress, molecular inflammation and sarcopenia. Article CAS PubMed PubMed Central Google Scholar.

Wu, L. Urinary 8-OHdG: a marker of oxidative stress to DNA and a risk factor for cancer, atherosclerosis and diabetics. Clin Chim Acta. Ferreira, P.

Food Funct. Blood-brain barrier permeability of green tea catechin metabolites and their neuritogenic activity in human neuroblastoma SH-SY5Y cells. Food Res. Shimizu, C. Moderate-dose regular lifelong alcohol intake changes the intestinal flora, protects against aging, and keeps spatial memory in the senescence-accelerated mouse prone 8 SAMP8 model.

Lozupone, C. UniFrac: an effective distance metric for microbial community comparison. ISME J. von Hertzen, L. Natural immunity. Biodiversity loss and inflammatory diseases are two global megatrends that might be related.

EMBO Rep. Ley, R. Microbial ecology: human gut microbes associated with obesity. Wakita, Y. Taxonomic classification for microbiome analysis, which correlates well with the metabolite milieu of the gut.

Arumugam, M. Enterotypes of the human gut microbiome. Wu, G. Linking long-term dietary patterns with gut microbial enterotypes. Henning, S. Decaffeinated green and black tea polyphenols decrease weight gain and alter microbiome populations and function in diet-induced obese mice.

Yamamoto, K. Japan patent JP B2 registration date: January 13, Hosokawa, M. Grading score system: a method for evaluation of the degree of senescence in senescence accelerated mouse SAM. Murai, T. Characteristics of object location memory in mice: Behavioral and pharmacological studies.

Matsuki, T. Quantitative PCR with 16S rRNA-gene-targeted species-specific primers for analysis of human intestinal bifidobacteria. Appl Environ Microbiol. Kim, S. Robustness of gut microbiota of healthy adults in response to probiotic intervention revealed by high-throughput pyrosequencing.

DNA Res. Download references. We thank Ms. Izumi Nomura, Frontier Laboratories for Value Creation, SAPPORO HOLDINGS LTD.

Frontier Laboratories for Value Creation, SAPPORO HOLDINGS LTD. Foodtechnology Laboratories for Value Creation, SAPPORO HOLDINGS LTD, Nippa-cho, Kouhoku-ku, Yokohama, , Japan. Fujita Health University, Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, , Japan.

NPO Japanese Drug Organization of Appropriate Use and Research, Omoteyama, Tanpaku-ku, Nagoya, , Japan. You can also search for this author in PubMed Google Scholar.

and Y. designed the study and performed experiments. provided technical support and conceptual advice. supervised experiments and provided critical revision for the manuscript. Correspondence to Chikako Shimizu.

are employees of SAPPORO HOLDINGS LTD. is a retiree of SAPPORO HOLDINGS LTD. has consulted for SAPPORO HOLDINGS LTD. and received compensation. Open Access This article is licensed under a Creative Commons Attribution 4. Reprints and permissions. Effects of lifelong intake of lemon polyphenols on aging and intestinal microbiome in the senescence-accelerated mouse prone 1 SAMP1.

Sci Rep 9 , Download citation. Received : 12 October Accepted : 12 February Published : 06 March Anyone you share the following link with will be able to read this content:.

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Also known as Kakadu plum, a native Australian superfood with antioxidant, healing and protective properties. Anti-G-Ox is a daily essential for anyone with modern skin needs wanting to fight premature ageing.

That is, if you live in a city. Have a demanding schedule. Spend a little too much time in the sun. Not enough time sleeping. Or your skin struggles after an indulgent meal. During this time, you should talk to a trusted healthcare professional before taking Anti-G-Ox — or any supplement, that is.

Anti-G-Ox is an effervescent. This means it fizzes and dissolves instantly when in contact with moisture of any kind. Like the water in your glass or the moisture on your tongue.

Effervescents are fully dissolved by the time they reach the stomach. So your body can absorb them faster and more completely.

Clinical studies have even shown that ingredients in effervescent formulas enter the bloodstream in just minutes. While rare in ingestible products, some people may have an allergic reaction with plant extracts.

If you experience any allergy symptoms after taking Anti-G-Ox, we recommend consulting a healthcare practitioner before continuing use. Anti-G-Ox also contains mannitol, a sugar alcohol and a potential allergen. Anti-G-Ox is free from animal products.

Shop Anti-G-Ox Citrus. Chevron icon left Chevron icon right. Anti-G-Ox Citrus. Best value. Select delivery frequency DELIVER EVERY 14 DAYS DELIVER EVERY 30 DAYS DELIVER EVERY 60 DAYS DELIVER EVERY 90 DAYS.

Quantity Decrement icon Increment icon. Berry Citrus. Add to Bag. Boost your routine Smooth and protect. We recommend adding Natural Marine Collagen to advance your beauty regime. Close icon PayPal - Pay in 4 Shop Pay Installments Pay In 4: Easy as Make the first payment at the time of purchase and pay the rest in 3 payments - one every two weeks.

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Returns Vida Glow is designed for real, active people. Suitable for vegans. Actives Nutritional info Vitamin C An antioxidant powerhouse that fights free radicals and promotes collagen health. Nicotinamide A powerful antioxidant also known as niacinamide that maintains healthy collagen, supports cellular repair and assists healthy sugar metabolism.

Vitamin B6 An essential vitamin that supports healthy sugar metabolism, cell growth and repair. Biotin A beauty-enhancer that helps the body metabolise nutrients. Magnesium An essential element that supports overall wellbeing.

Zinc An antioxidant mineral that's essential for healing wounds and maintaining healthy skin. Chromium A powerful trace mineral that supports healthy blood sugar levels. Curcumin A skin-strengthening antioxidant compound found in turmeric. Terminalia ferdinandiana Also known as Kakadu plum, a native Australian superfood with antioxidant, healing and protective properties.

How to use Mix in water or take straight on the tongue. Product FAQs If your question isn't answered here and you'd like to speak to someone, we are here to help.

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Woman Almost Dies after Taking Daily Supplements? Oranges Citgus long been acclaimed for their high Muscle recovery strategies C content and subsequent immune boosting properties, Ctrus there Citrus supplement for anti-aging Citrsu to the sweet Citrus supplement for anti-aging anti-agung fruit than meets the eye. Along with other citrus fruit varieties, they GI and energy levels fpr a dramatic role as anti Best water bottles for camping foods for anti-agint seeking a helping hand in turning back the clock. Fight Age Related Disease Citrus fruit contains a host of bioflavonoids and other phytochemicals that have been shown in studies to provide anti-inflammatory and antioxidant properties. Arthritic illnesses as well as disorders where the immune system is challenged can all be helped through regular consumption of citrus fruit. This anti aging food also contains properties which have been shown to increase cell turnover so that older cells are encouraged to die off and be replaced by newer ones, a process that often slows with increased age. Citrus supplement for anti-aging

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